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phoenix 293t packaging cells  (ATCC)


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    ATCC phoenix 293t packaging cells
    Phoenix 293t Packaging Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38742 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phoenix 293t packaging cells/product/ATCC
    Average 99 stars, based on 38742 article reviews
    phoenix 293t packaging cells - by Bioz Stars, 2026-02
    99/100 stars

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    ( a ) Enrichment plot (green) of GSEA using expression profiles of human macrophages isolated from SF of RA ( n = 3) and synovial tissue of OA patients (n = 3). ( b ) GSEA as in a using expression profiles of human BMDC from RA ( n = 9) and OA ( n = 10) patients. The GSI_Notch gene set was used for GSEA ( a , b ). The bottom section shows the ranking metric value, which indicates gene correlation with a phenotype. ( c ) Relative HEY-1 mRNA levels in CD14 + cells from RA and OA patients. ( d ) miR-223 levels in HEK-293T cells overexpressing HEY-1 or an irrelevant cDNA (GFP; mock). ( e ) Determination of Notch3 mRNA levels in RA and OA isolated cells. ( f ) Linear regression analysis of Notch3 and miR-223 levels in RA and OA samples. Only samples with paired determinations of Notch3 and miR-223 were included in the analysis ( n = 11). ( g ) Relative mRNA levels of the Notch effector HES-1 in the indicated samples. ( h ) Monocytes healthy donors were co-cultured with mock or Notch ligand-expressing OP-9 cells in conditions without (transwell) or with cell-cell contact (co-culture), alone or with the Notch inhibitor DAPT. After cell sorting, monocyte miR-223 levels were determined by RT-qPCR. For ( c , e , f , g ) each point represents one patient. For ( d , h ), data are mean ± SEM from triplicate RQ determinations in at least 3 independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.

    Journal: Scientific Reports

    Article Title: Notch-regulated miR-223 targets the aryl hydrocarbon receptor pathway and increases cytokine production in macrophages from rheumatoid arthritis patients

    doi: 10.1038/srep20223

    Figure Lengend Snippet: ( a ) Enrichment plot (green) of GSEA using expression profiles of human macrophages isolated from SF of RA ( n = 3) and synovial tissue of OA patients (n = 3). ( b ) GSEA as in a using expression profiles of human BMDC from RA ( n = 9) and OA ( n = 10) patients. The GSI_Notch gene set was used for GSEA ( a , b ). The bottom section shows the ranking metric value, which indicates gene correlation with a phenotype. ( c ) Relative HEY-1 mRNA levels in CD14 + cells from RA and OA patients. ( d ) miR-223 levels in HEK-293T cells overexpressing HEY-1 or an irrelevant cDNA (GFP; mock). ( e ) Determination of Notch3 mRNA levels in RA and OA isolated cells. ( f ) Linear regression analysis of Notch3 and miR-223 levels in RA and OA samples. Only samples with paired determinations of Notch3 and miR-223 were included in the analysis ( n = 11). ( g ) Relative mRNA levels of the Notch effector HES-1 in the indicated samples. ( h ) Monocytes healthy donors were co-cultured with mock or Notch ligand-expressing OP-9 cells in conditions without (transwell) or with cell-cell contact (co-culture), alone or with the Notch inhibitor DAPT. After cell sorting, monocyte miR-223 levels were determined by RT-qPCR. For ( c , e , f , g ) each point represents one patient. For ( d , h ), data are mean ± SEM from triplicate RQ determinations in at least 3 independent experiments. *p < 0.05, **p < 0.01, Mann-Whitney U -test.

    Article Snippet: Individual retroviral constructs were transfected into the packaging 293T Phoenix-Amphotropic cell line (provided by G. Nolan, Stanford University, Stanford, CA, USA), and supernatants obtained from puromycin-selected transfected cells were used to transduce OP-9 cells (ATCC) by centrifugation in the presence of polybrene.

    Techniques: Expressing, Isolation, Cell Culture, Co-Culture Assay, FACS, Quantitative RT-PCR, MANN-WHITNEY